聚合酶链反应（PCR）的开发旨在找出病毒小片段，然后进行扩增，产生多个拷贝，使其更容易被检测到。聚合酶链反应是检测2019冠状病毒病和其他病毒性疾病（如埃博拉）的黄金标准。了解这项核衍生技术的工作原理。

本研究针对猫传染性腹膜炎（FIP）诊断中传统RT-qPCR技术依赖昂贵设备、需外送检测的局限性，开发了两种基于STH-PAS（单标签杂交印迹阵列条）的快速检测系统：RT-PCR-STH-PAS与RT-RPA-STH-PAS。研究证实两种系统检测限分别达104.2和104 copies/reaction，临床验证灵敏度66.7% ...

为解决登革热实验室诊断缺乏共识标准的问题，研究人员通过贝叶斯随机效应模型对RT-PCR、NS1 ELISA和IgM ELISA三种检测方法进行系统评价。结果显示NS1 ELISA在症状出现0-4天的灵敏度达90%（95% CrI 68-98），与RT-PCR（95%）相当，而IgM ELISA在早期灵敏度仅17%。该研究为资源 ...

A new preprint describes a single-step molecular diagnostic test for the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection caused by the Omicron variant that is behind ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product accumulation, ...

Respiratory syncytial virus (RSV) was detected easiest when using reverse transcription polymerase chain reaction (RT-PCR) tests in a pediatric population. Reverse transcription polymerase chain ...

During the early stages of the coronavirus disease 2019 (COVID-19), rapid testing was imperative for public health policymakers and health care workers in order to determine where and what measures ...

Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

At-home COVID-19 tests have become an easy way to self-diagnose. But current tests have drawbacks, such as the length of time it takes to get an answer, or how accurately the test can identify a ...