Sections were incubated in 3, 3'-Diaminobenzidine, Tris-buffered saline, H 2 O 2 for <5 min. Sections were mounted and coverslipped (Cytoseal, Thermo Fisher Scientific, Grand Island, NY, USA). Images ...

2.2 Microscopy and image acquisition Morphophysiological characteristics of the culture were examined using Zeiss Axioplan 2 microscope equipped with Axiovision 4.2 software (Zeiss, Germany) at × ...