Tissue Processing | Lab Tests Guide
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Tissue Processing | Lab Tests Guide
The technique of getting fixed tissue into paraffin is called tissue processing. Tissue processing is designed to remove all extractable water from the tissue, replacing it with a support medium that provides sufficient rigidity to enable sectioning of the tissue without damage or distortion
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In histopathology, cutting of frozen sections is a rapid tissue processing technique mainly used for intraoperative diagnosis (to guide surgeons during surgery). Instead of the usual paraffin embedding (which takes hours to days), the frozen section method allows diagnosis within minutes. Here’s a breakdown: --- Principle Fresh tissue is rapidly frozen to preserve its structure. The frozen tissue becomes firm enough to be cut into very thin sections using a special microtome (cryostat). Sections
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