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视频: 实时 RT-PCR
62.5K 次观看. 实时逆转录聚合酶链反应或实时 RT-PCR 是一种用于确定靶基因表达水平的分析工具。该方法涉及在称为逆转录酶的酶的帮助下将 mRNA 转化为互补 DNA,然后对 cDNA 进行 PCR 扩增。这两个过程可以在单管中同时进行,也可以作为两步反应单独进行 ...
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